High-standard Technological Services (STANs)

STAN Specifications: Analysis of fatty acids by gas chromatography.
Methodology: Analysis by CGC: An Omega Wax 250 column of 30 m will be used.
The operative conditions will be as follows: injector in split mode at 260 260°C, programmed oven temperature, and flame ionization detector will be maintained at 280°C. Helium will be the gas carrier at a flux of 1-2 ml/min.
Equipment: Gaseous Chromatograph Hewlett Packard 6890.
Primary Discipline: Biochemistry.
Secondary Discipline: Analytical Chemistry.
Area of application: Hygiene, diet and nutrition. Others.
Industrial Activity: Assays and technical analysis.

STAN Specifications: Analysis by gaseous chromatography – mass spectrometry for the detection / identification of compounds or organic contaminants.
Methodology: 1) Determination of optimal operational conditions of sample nature. Sample taking (liquid or gaseous) and injection. Deliver of chromatogram and spectra of interest in printed or electronic version.
2) Identity assignment by comparison of spectra obtained with spectra commercial libraries (NIST 05/EPA/NIH, pesticides, essential oils and others) and with other spectra obtained from standards (provided by the applicant).
Equipment: Capillar gas chromatograph with spectrometry detector of mass Agilent CGC-HP6890 MS5975VL.
Primary Discipline: Biochemistry.
Secondary Discipline: Analytical Chemistry.
Area of application: Hygiene, diet and nutrition. Other
Industrial Activity: Assays and technical analysis.

STAN Specifications: Fractioning of biological samples by differential ultracentrifugation.
Methodology: The fractioning of biological samples will be performed previously to adequate rotor selection and rotation velocity, solvent density, etc.
Installation startup, sample loading, and ultracentrifugation.
Equipment: Beckman Coulter LE-80K Preparative Ultracentrifuge.
Primary Discipline: Biochemistry.
Secondary Discipline: Biology.
Area of application: General Knowledge Promotion. Natural and Exact Sciences.
Industrial Activity: Assays and technical analysis.

STAN Specifications: Evaluation of insecticide capacity. Analysis of insecticide efficacy of biological, physical or chemical insecticide agents by means of mortality determination, effect on life cycle of insects, etc, and/or evaluation of lethal doses.
Methodology: Bioassays will be performed by exposing insects to compounds to be evaluated. The application mode depends on the physicochemical properties of the compound and/or as it was solicited. Batches of at least 10 insects will be employed, with at least 4 replicas for each concentration or condition to be assayed. Statistical analysis of the results.
Equipment: 
Primary Discipline:  Biology.
Secondary Discipline: Biology - Entomology.
Area of application: Vegetal Health – Plagues.
Industrial Activity: Cereal farming except forage crop and seeds (Including buckwheat, malting barley, etc.)
Farming of pome fruits (Including quince, loquat, etc.)

STAN Specifications: Protein purification by molecular exclusion and ion exchange.
Methodology: 1)Protein purification by ion Exchange: It is used an ion exchanger column (Mono-Q 10/100 GL) that separates proteins based upon its superficial net charge at a determined pH value. The different gradients of increasing ionic strength affect the interaction between the filling and the biomolecules, thus achieving their separation. Protein detection is performed by absorbance at 280 nm. Brenner 2)Protein purification by molecular exclusion: It is used molecular exclusion columns capable of separating globular proteins in a range of molecular masses between 3,000 and 70,000 Da (Superdex 75 10/300GL) and between 100,000 and 600,000 Da (Superdex 200 10/300 GL). The mobile phase is an aqueous buffer which is isocratically used. The presence of proteins in the column eluent is detected by absorbance at 280 nm.
Equipment: 
Primary Discipline: Biochemistry and Molecular Biology.
Secondary Discipline: Biology - Biochemistry.
Area of application: General Knowledge Promotion – Natural and Exact Sciences.
Industrial Activity: Research and experimental development in the field of natural and exact sciences n. c. p. (not yet classified for the Argentine Tax Agency, AFIP).

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Primary Discipline: Medical sciences.
Secondary Discipline: Biology - Cellular and molecular.
Area of application: Human health.
Industrial Activity: Research and experimental development in the field of medical sciences.

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Primary Discipline: Biochemistry and Molecular Biology.
Secondary Discipline: Biology - Cellular and molecular.
Area of application: General Knowledge Promotion – Natural and Exact Sciences.
Industrial Activity: Assays and technical analysis.

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STAN Specifications: The service consists of processing biological samples of human, animal or vegetal origin, cellular lines or microorganisms by means of the acquisition in the Accuri C6 Plus (BD) flow cytometry.
Appointments will be made through the Federal System of Flow Cytometry www.argentina.gob.ar/ciencia/sact/sistema-de-gestion-de-turnos
Methodology: Before sample processing, control quality of the equipment will be performed with RUO beads for calibration. Signaling compensation will be carried out (if necessary). Populations of interest will be determined and samples will be analyzed according to the present fluorophores (2 lasers, 6 parameters available).

Equipment specifications

Excitation: blue laser (488nm), red laser (640nm).
Detectors: size (FSC) and inner complexity (SSC) plus four detectors independent of fluorescence.
Configuration and predefined filters of fluorescence detection: Configuration 3 blue (FL1, FL2, FL3): 1 red (FL4).
Filters: 533/30 nm (FL1), 585/40 nm (FL2), 670 LP nm (FL3), 675/25 nm (FL4).
Software for acquisition and analysis of data: BD Accuri (BD Biosciences).
Configurations and additional filters: Configuration 4 blue (FL1-4): 0 red - 2 blue (FL1, FL2): 2 red (FL3, FL4) Filters 510/15 nm, 540/20 nm, 565/20 nm, 780/60 nm, 610/20 nm, 630/30 nm
The analysis of the acquired parameters is not included in the basic service, but it can be coordinated with the user.
Info:  citometro@med.unlp.edu.ar
Equipment: Citómetro de flujo Accuri C6Plus (BD).
Primary Discipline: Bioquímica y Biología Molecular.
Secondary Discipline: Biología - Celular y Molecular.
Area of application: Prom. Gral. del Conoc. -Cs. Exactas y Naturales.
Industrial Activity: Ensayos y análisis técnicos